Production of fatty acid from palm oil by immobilized lipase
Wipawadee Saetan, D. of Industrial Biotech., F. of Agro-Industry, PSU.
Poonsuk Prasertsan, Assoc. Prof., D. of Industrial Biotech., F. of Agro-Industry, PSU.
Aran H-Kittikun, Asst. Prof., D. of Industrial Biotech., F. of Agro-Industry, PSU.
Corresponding e-mail : haran@ratree.psu.ac.th
Grant : TRF
Presented : The 12th Annual Meeting of the Thai Society for Biotechnology "Biotechnology :
Impacts & Trends" Kanchanaburi, Thailand, 1-3 Novermber, 2000
Key words : fatty acid, immobilized lipase, packed-bed reactor, lipase AY, Candida rugosa
Lipase AY from Candida rugosa possessed hydrolytic activity against palm oil with specific lipase activity of 599.41 U/mg protein. This enzyme had the optimum temperature and pH for hydro-lysis at 45oC and 7, respectively. Immobilization of lipase AY by physical adsorption on Accurel EP 100 (<400 mm) was used to hydrolyze palm oil. The Accurel was pretreatment by ethanol before use. The optimum conditions for immobilization were 10 ml (3 mg/ml) enzyme in 0.2 M phosphate buffer pH 7 mixed with Accurel 100 mg at 4oC for 3 h. Under these conditions, activity of immobilized li-
pase was 640 units/g Accurel. Optimal conditions for hydrolysis of palm olein by immobilized lipase were observed with 0.03 g immobilized enzyme in 4 ml reaction mixture (10% palm olein in 2% gum arabic solution) at 45oC and a shaker speed of 250 rpm. The degree of hydrolysis was 82% after 24
h and immobilized lipase lost 50% of its activity after recycling 4 times. Study on the continuous hydrolysis of palm olein was done with immobilized lipase 960 units in the packed-bed reactor (PBR) (Æ0.62x20 cm) at 45oC. The feeding rate of the substrate was 0.05 ml/min. In a packed-bed reactor
of immobilized lipase the maximum degree of hydrolysis was 86% after 96 h and the immobilized
lipase lost 50% of its activity after 288 h.
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