Histology of callus formation in rice (Oryza sativa L.) seed culture
เนื้อเยื่อวิทยาของการเกิดแคลลัสจากการเพาะเลี้ยงเมล็ดข้าว (Oryza sativa L.)

Benjawan Yanvisadepakdee, D. of Biology, F. of Sci., PSU.
Upatham Meesawat, D. of Biology, F. of Sci., PSU.
Kamnoon Kanchanapoom, Assoc. Prof., D. of Biology, F. of Sci., PSU.
Corresponding e-mail : kkamnoon@ratree.psu.ac.th

Grant : Faculty of Science, PSU.
Published : Songklanakarin J Sci Technol 2000, 22(1) : 1-6
Key words : callus, histology, Oryza sativa L., rice, seed culture

Seeds of rice (Oryza sativa L.) cultivar Khao Dawk Mali 105 were cultured on LS (Linsmaier and Skoog, 1965) medium supplemented with 2 mg/l 2,4-D and 0.5 mg/l kinetin. Callus was initiated from these seeds within 7 days. The cultured seeds were harvested at various times, fixed, sectioned, stained and examined microscopically. The histological origin of the callus revealed that callus formation occurred from cells with dense cytoplasm in vascular tissue of mesocotyl and epithelium tissue of scutellum.

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