A lectin from the latex of Heavea brasiliensis : purification and characteri- zation

Rapepun Wititsuwannakul, Assoc. Prof., D. of Biochemistry, F. of Sci., PSU.
Piyaporn Pasitkul, D. of Biochemistry, F. of Sci., PSU.
Dhirayos Wititsuwannakul, Assoc. Prof., D. of Biochemistry, F. of Sci., Mahidol U., Bangkok
Kamonchanok Rukseree, D. of Biochemistry, F. of Sci., Mahidol U., Bangkok
Bruce A. Stone, D. of Biochemistry, La-Trobe U., Melbourne, Australia
Corresponding e-mail : wrapepun@ratree.psu.ac.th

Grant : Government Budget
Published : Research Report
Key words : Hevea brasiliensis, Euphobiaceae, rubber latex, lectin, membrane

A protein extracted from an acetone precipitated fraction, prepared from the bottom or lutoid body-riched fraction of centrifuged fresh lates, in a buffer containing 0.2% Triton X-100. It was able to agglutination erythrocytes from either rabbit or mouse but not human. The Hevea latex lectin (HLL) was purified to homogeneity by chitin batch-binding and DEAE-Sepharose column. Its Mr upon SDS-PAGE of is 17 kD with native M_r obtained by gel filtration of 276 kD. It has a pl value of 5.1 and pH stability ranging from 5-10. It is heat stable up to 60^oC and activated by low Ca²⁺ concentration. The hemagglutination of HLL was inhibited by several glycoproteins such as fetuin, asialofetuin, ovomucoid, mucin, asialomucin, type II-S trypsin inhibitor but not a1-acid glycoprotein, soybean trypsin inhibitor and mono-, di- or tri-saccharides.

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