Molecular phylogenetic studies in Zingiberaceae with particular reference to Boesenbergia Kuntze, and related genera

Molecular phylogenetic studies in Zingiberaceae with particular reference to Boesenbergia Kuntze, and related genera
การจำแนกพืชวงศ์ขิงในสกุลกระชายและสกุลที่เกี่ยวข้องโดยวิธีชีววิทยาระดับโมเลกุล

Ongkarn Vanijajiva, M.Sc. Student in Biochemistry, PSU.
Wallie Suvachittanont, Assoc. Prof., D. of Biochemistry, F. of Sci., PSU.
Puangpen Sirirugsa, Prof., D. of Biology, F. of Sci., PSU.
Corresponding e-mail : saxx9@hotmail.com

Grant : Graduate Studies PSU, Biodiversity Research and Training Program
Presented : The 3rd Regional IMT-GT Uninet Conference IMT-GT 2000, 10-12 October 2000, Medan Indonesia
Key words : Zingiberaceae, Boesenbergia , isozyme, phylogenetic

Phylogenetic relationship of Zingiberaceae species with the emphasis on Boesenbergia has been investigated using molecular markers, namely isozyme patterns and DNA analysis. In present study, only isozyme electrophoresis results of leaves extracts from 9 species of Boesenbergia (B.basispicata, B.curtisii, B.prainiana, B.plicata, B.pulchellun, B. pulcherrima, B.longipes, B.rotunda, B.sp.), 6 species of Kaempferia (K.angustifolia, K.elegans, K.galanga, K.pulcha, K.siamensis, K. sp) and 2 species of Scaphochamys (S.biloba, S.sp.) are reported. Nine enzyme systems were used in this study. These included peroxidase (POX), superoxide dismutase (SOD), glutamate dehydro- genase (GDH), malate dehydrogenase (MDH), shikimic dehydrogenase(SKD), a-esterase (a-EST), b-esterase (b-EST), acid phosphatase (ACP) and alkaline phosphatase (AKP). Only 4 enzyme systems (POX, SOD, GDH, MDH) appeared to be useful as molecular markers for characterization of Boesenbergia and related genera. Our preliminary result from 2 sets of extraction of leaves samples and 3 sets of isozyme electrophoresis run showed that POX and SOD are useful in identifying Boesenbergia and Kaempferia. GDH and MDH are only useful for Kaempferia . Other isozyme systems did not give sufficient resolution and band patterns were inconsistent. Attempts are being made to obtain good and reproducible isozyme patterns in other enzyme systems. At this stage, it is promising that the technique is useful for identifying Boesenbergia from other species. It is hoped that morphological data and isozyme electrophoressis data are useful for a better understanding of Zingiberaceae systematic, evolution and biodiversity as a whole.

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