Dolichols of rubber plant, ginkgo and pine
Seiji Tateyama, Institute for Chemical Raction Science, Tohoku U., Aoba-ku, Japan
Rapepun Wititsuwannakul, Assoc. Prof., D. of Biochemistry, F. of Sci., PSU.
Dhirayos Wititsuwannakul, Assoc. Prof., D. of Biochemistry, F. of Sci., Mahidol U., Bangkok
Hiroshi Sagami, Institute for Chemical Raction Science, Tohoku U., Aoba-ku, Japan
Kyozo Ogura, Institute for Chemical Raction Science, Tohoku U., Aoba-ku, Japan
Corresponding e-mail : yasagami@icrs.tohoko.ac.jp
Published : Phytochemistry 1999, 51 : 11-15
Key words : polyprenol, dolichol, ginkgo, Ginkgo biloba, ginkgoaceae, pine, Pinus sylvestris,
pinaceae, Hevea brasiliensis, Euphobiaceae, rubber, two-dimensional thin-layer
chromatography
Using a two-plate thin-layer chromatography method, we analyzed polyisoprenoid alcohols (dolichols and polyprenols) of the rubber plant Hevea brasiliensis (angiosperm), and of ginkgo
Ginkgo biloba and pine Pinus sylvestris (gymnosperms). Special attention was paid to the occurrence of dolichol in various tissues of different plants. Dolichols were found to occur in all of the tissues examined except for flowers of the rubber plant. The chain length distributions of dolichols in seeds, young roots, young shoots, young leaves and old leaves of the rubber plant were C70-C95, C85-C105,
C80-C105 and C65-C90, respectively. In the case of ginkgo, the chain length distributions of dolichols in seeds, embryos, young and old leaves were C70-C90, C70-C85, C70-C90 and C80-C95, respectively. Pine seeds were found to contain dolichols with the chain length distribution of C70-C90. Two kinds of polyprenol families were detected in leaves of the rubber plant and ginkgo. The longer chain poly-prenol family was also detected in seeds of the rubber plant, in seeds and embryos of ginkgo and in
seeds of pine. The chain length distributions of the polyprenols were not necessarily the same as those of dolichols occurring in the same tissues.
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