Swimming behaviour and motility of the buck spermatozoa

Swimming behaviour and motility of the buck spermatozoa
การศึกษาลักษณะการเคลื่อนที่และการเคลื่อนไหวของตัวอสุจิแพะ

Peerasak Suttiyotin, Asst. Prof., D. of Animal Sci., F. of Natural Resources, PSU.

Grant : Government Budget
Published : Research Report
Key words : buck spermatozoa, goat spermatozoa

Swimming behaviour and motility of goat spermatozoa were studied in semen diluted with Tris base diluent. Images of swimming pattern (400X) were recorded with the aid of a video recorder connected with a microscope. The images were later playbacked for motility evaluation. The images were also displayed frame by frame and tracks of spermatozoa moving were drawn on a transparancy attached to a monitor screen. Sperm velocity was calculate from path of traveling against time.

Track velocity, mean path velocity and progressive velocity infresh semen were 135.7ฑ2.8, 49.6ฑ1.1 and 44.7ฑ1.1 micron/sec respectively. The percentage of motile cells, progressive motile cells and arch motile cells in fresh semen were 70.6ฑ3.5, 55.0ฑ4.4 and 13.8ฑ3.1% respectively.

Cold shock for 0 to 3 min had no effect on track velocity (155.1ฑ18.8 to 165.7ฑ17.1 micron/ sec) and path velocity (44.2ฑ2.5 to 48.8ฑ2.6 micron/sec). The progressive velocity in control group, however, was significantly lower than that of cold shocked (52.8ฑ1.5 VS 61.5ฑ2.5 micron/sec, p<0.01)

The percentages of motile cells decreased with increasing time of cold exposure and it decreased from 77.5ฑ2.5% in control group to 67.5ฑ2.5 (p<0.05), 45.0ฑ2.9 (p<0.01) and 4.00ฑ0.0 (p<0.01) in semen cold shocked for 1, 2 and 3 min respectively. The percentages of spermatozoa swimming in straight direction were started to show significant effects when semen was exposed to cold shock for 2 and 3 min (27.5ฑ15.0 and 35.0ฑ4.1% respectively) when compared to control group (71.3ฑ7.2%, p<0.01). The percentages of spermatozoa swimming in curve, however, had little effects from the shock.

Hot shock for upto 5 seconds had no effects on track velocity, path velocity and progressive velocity. However, exposure of semen to hot shock for 0 to 5 sec had no effects on track velocity (range from 106.6ฑ2.7 to 125.2ฑ15.7 micron/sec), path velocity (28.0ฑ1.6 to 34.1ฑ1.5 micron/sec) and progressive velocity (21.6ฑ1.6 to 26.6ฑ1.3 micron/sec). The percentages of motile spermatozoa decline with increasing time of hot exposure and it decreased from 57.5ฑ2.5% in control group to 40.0ฑ0.0, 25.0ฑ2.7 and 11.3ฑ3.1% (all p<0.01) in semen hot shocked for 1, 2 and 5 sec respectively. The percentages of direct swimming spermatozoa also decreased 31.3ฑ6.6% in control group to 13.8ฑ 1.3% (p<0.05), 3.8ฑ1.3% (p<0.01) in semen hot shocked for 2 and 5 sec respectively. The percentages of curve swimming spermatozoa, however, had little effects from hot shock, ranged from 7.5ฑ2.5 to 23.8ฑ6.3%

In general, sperm velocity declined with increasing incubation time from 1 to 3 hours. The tracks velocity declined from 120.2ฑ4.0 micron/sec in control group to 99.9ฑ2.7% in semen incubated for 1 hour (p<0.01) but the value for those of 2 and 3 hours (114.1ฑ8.0 and 108.3ฑ3.3 micron/sec respec- tively, p>0.05) were not significantly different from the control. For path velocity, it decreased from 46.3ฑ1.5 micron/sec in control group to 34.9ฑ1.6, 40.2ฑ1.6 and 35.0ฑ1.6 micron/sec in semen incu-bated for 1, 2 and 3 hours respectively (all p<0.01). The progressive velocity decreased in the same manner from 39.8ฑ1.8 micron/sec in control group to 29.7ฑ1.5 (p<0.01), 35.1ฑ1.6 (p<0.05) and 29.7ฑ 1.7 (p<0.01) micron/sec in semen incubated for 1, 2 and 3 hour respectively.

The percentages of motile spermatozoa started to show significant decline when semen reached incubation time of 2 and 3 hour (17.5ฑ4.8 and 17.5ฑ2.5% respectively, p<0.01) when compare to the control (70.0ฑ4.1%). The percentages of direct swimming spermatozoa showed similar reduction and it decreased from 53.8ฑ4.7% in control group to 10.0ฑ3.5 and 15.0ฑ3.5% in semen incubated for 2 and 3 hours respectively (all p<0.01). The percentages of curve swimming spermatozoa also decreased from 16.3ฑ2.4% in control group to 5.0ฑ2.0% (p<0.05) and 2.5ฑ1.4% (p<0.01) in semen incubated for 2 and 3 hours respectively.

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