Immunostimulant and vaccination in black tiger shrimp, Penaeus monodon Fabricius : III The effect of b-glucan (MacroGard า) on growth performance, immune response and disease resistance in black tiger shrimp, Penaeus
monodon Fabricius
สารกระตุ้นภูมิคุ้มกันและการใช้วัคซีนในกุ้งกุลาดำ : III ผลของบีต้ากลูแคน (MacroGardา) ต่อการเจริญเติบโต ภูมิคุ้มกัน และความต้านทานโรคในกุ้งกุลาดำ
Kidchakan Supamattaya, Assoc. Prof., D. of Aquatic Sci., F. of Natural Resources, PSU.
Juadee Pongmaneerat, Department of Fisheries, Kaset Klang Bangkhen, Jatujak, Bangkok
Thanawut Klowklieng, Coastal Aquaculture Development Center, Satun
Corresponding e-mail : skidchak@ratree.psu.ac.th
Grant : Biotec ASA, Norway and BIOTEC
Published : Songklanakarin J Sci Technol 2000, 22(Suppl.) : 677-687
Key words : Immunostimulant, b-glucan, disease resistanc, black tiger shrimp,
Penaeus monodon
The effects of an immunostimulant "b-glucan (MacroGardา)" was carried out using three
shrimp sizes with average weight of 0.6, 1.5 and 6.5 g for 3 experimental groups, respectively. Shrimps were fed diets containing MacroGardา at 0, 0.25, 0.50 and 1.0 g/kg feed for 11 weeks. The effect of MacroGardา on growth and feed performances were observed from experiment 1, for group feeding trials. The results showed that supplementation of MacroGardา at different levels in diets had no effect on growth, survival and feed conversion of shrimp.
Resistance to pathogenic bacteria (Vibrio harveyi) was assessed after both small and large sizes of experimental shrimp received test diet for 6 and 5 weeks, respectively. Increasing levels of the immunostimulant in the diet resulted in increasing survival rate of shrimp in both sizes. Survival of shrimp receiving MacroGardา at 0, 0.25, 0.50 and 1.0 g/kg feed were 50, 68, 69 and 81% in small
shrimp and 33, 33, 60 and 73% in large shrimp, respectively.
Both groups of shrimp that received MacroGardา at 1.0 g/kg feed showed the highest total hemocyte count and superoxide anion production.
Bacterial clearance ability of shrimp after receiving MacroGardา for different periods was also tested. After 6 weeks, the shrimp receiving the MacroGardา at 1.0 g/kg feed showed the highest effi-ciency of clearance ability. Whereas, shrimp receiving MacroGardา at lower levels showed similar results. After 11 weeks feeding, clearance ability of shrimp receiving all levels MacroGardา of was similar and higher than that of the control group.
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