TThe immune system in black tiger shrimp, Penaeus monodon, Fabricius :
IV. Normal immuno-physiological values in black tiger shrimp, Penaeus
monodon, Fabricius
ระบบภูมิคุ้มกันโรคในกุ้งกุลาดำ : IV. การศึกษาค่าปกติของระบบภูมิคุ้มกันและองค์ประกอบ เลือดในกุ้งกุลาดำ
Kidchakan Supamattaya, Assoc. Prof., D. of Aquatic Sci., F. of Natural Resources, PSU.
Jareeporn Ruangsri, Aquatic Animal Health Research Center, F. of Natural Resources, PSU.
Suphada Kiriratnikom, Aquatic Animal Health Research Center, F. of Natural Resources, PSU.
Narin Songsrichan, Aquatic Animal Health Research Center, F. of Natural Resources, PSU.
Corresponding e-mail : skidchak@ratree.psu.ac.th
Grant : BIOTEC and Government Budget
Published : Songklanakarin J Sci Technol 2000, 22(Suppl.) : 597-603
Key words : immuno-physiology, blood parameter, immune system, hemocyte,
black tiger shrimp, Penaeus monodon
A comparative study was carried out to determine the variations of blood parameters, i.e. total hemocyte count, plasma protein, blood sugar, plasma pH, plasma osmolarity, plasma ion, phenoloxidase and phagocytic activity of hemocytes in cultured as well as in laboratory maintained shrimps. It was noted that the blood parameters varied over wide ranges. When comparing between the individuals from the farm and the laboratory environment, the total hemocyte counts were 6.49ฑ2.33 x 104 and 3.07ฑ1.91 x 104 cell/mm3 while phenoloxidase activity were 335.18ฑ106.29 and 217.84ฑ161.99 unit/min/mg protein, respectively. It indicates that variation in both blood parameters is influenced by culture environment particularly the temperature in the laboratory which may fluctuate widely as compared to the farm environment. Other blood parameters did not show significant differences between the two shrimp groups, i.e. plasma pH was 7.43ฑ0.15 and 7.78ฑ0.08 for the farm cultured
and laboratory-maintained individuals. The blood sugar for the laboratory-maintained individuals
had an average of 53.87ฑ54.84 mg% which was higher than that of the farm which had 32.91ฑ27.95 mg%. The serum protein showed no difference between the two shrimp groups, i.e. 11.85ฑ5.17 and 11.43ฑ3.81 g%. The osmolarity of plasma in cultured individuals was 778.49ฑ60.80 mOsmole/kg while the concentrations of Na+, K+, Cl- and albumin from plasma in the cultured individuals were 314.52ฑ28.96, 9.61ฑ1.20, 347.20ฑ45.54 mOsmole/kg and 1.51ฑ0.35 g%, respectively.
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