The immune system in black tiger shrimp, Penaeus monodon Fabricius
III. Electron microscopic studies on hemocytes of black tiger shrimp
(Penaeus monodon Fabricius)
ระบบภูมิคุ้มกันโรคในกุ้งกุลาดำ : III. การศึกษาทางจุลทรรศน์อิเลคตรอนของเม็ดเลือด
กุ้งกุลาดำ
Kidchakan Supamattaya, Assoc. Prof., D. of Aquatic Sci., F. of Natural Resources, PSU.
Suparb Kiattaptew, D. of Stomatology, F. of Dentistry, PSU.
Rudolf Hoffmann, Prof., Inst. of Zoology, Fisheries Biology and Fish Diseases, Germany
Corresponding e-mail : skidchak@ratree.psu.ac.th
Grant : BIOTEC and Government Budget
Published : Songklanakarin J Sci Technol 2000, 22(Suppl.) : 589-596
Key words : electronmicroscope, hemocyte, black tiger shrimp, Penaeus monodon
Hemocytes of black tiger shrimp (Penaeus monodon) were studied under electron microscopy. Three types of hemocytes were described : hyaline cell, semigranular hemocyte and large granular hemocyte. Hyalin cells were discoid, spindle or crescent shaped approximately 6.8-13.9 mm x 6.4-8.3 mm. Cells contained a large nucleus with scanty cytoplasm. Semigranular hemocytes were approximately 9.0-14.2 mm x 4.2-6.8 mm, with small granules 0.5-1.0 mm in the cytoplasm. Pseudopodia were formed with a few microvilli on the surface of the cell. Large granular hemocytes were approximately 12.2-14.6 mm x 7.2-7.8 mm and contained abundant 1.0-1.5 mm dense granules in the cytoplasm. Pseudopodia were formed with numerous microvilli. Hemocyte culture showed that there were more semigranular and large granular hemocytes than hyaline cells spread and adhering on glass slides. Virus infected hemocytes revealed decreased pseudopodia, decreased dense granules and karyorrhexis.
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