The immune system in black tiger shrimp, Penaeus monodon Fabricius : II. Cells and tissues involved the removal of foreign particles in black tiger shrimp(Penaeus monodon Fabricius)
ระบบภูมิคุ้มกันโรคในกุ้งกุลาดำ : II. เซลล์และเนื้อเยื่อที่เกี่ยวข้องในการกำจัดสิ่งแปลกปลอม ในกุ้งกุลาดำ

Kidchakan Supamattaya, Assoc. Prof., D. of Aquatic Sci., F. of Natural Resources, PSU.
Wutiporn Phromkunthong, Assoc. Prof., D. of Aquatic Sci., F. of Natural Resources, PSU.
Chutima Tantikitti, Asst. Prof., D. of Aquatic Sci., F. of Natural Resources, PSU
Rudolf Hoffmann, Prof., Inst. of Zoology, Fisheries Biology and Fish Disease, Germany
Corresponding e-mail : skidchak@ratree.psu.ac.th

Grant : BIOTEC
Published : Songklanakarin J Sci Technol 2000, 22(Suppl.) : 581-588
Key words : immune system, shrimp hemocyte, black tiger shrimp, Penaeus monodon

Saccharomyces cerevisiae were used as the foreign particles for tissue and cell examination and monitoring of the cellular defense system in black tiger shrimp (Penaeus monodon Fabricius) using the total hemocyte count (THC) and histological techniques. From the experiment, the THC rapidly decreased within 1 hour post injection, from 3.36ฑ1.85 x 10⁴ cell/mm³ in control to 2.12ฑ1.96 x 10⁴ cell/mm3 in treatment trial. Histological study showed that the hemocytes and fixed phagocyte in the gill tissue, lymphoid organ, heart tissue, hepatopancreas and connective tissue were sensitive to the foreign particles, but could be detected very slightly in muscle and nervous tissue. The mecha-nism of defense started with yeast particles being attacked by a single hemocyte or fixed phagocyte, followed by an increasing number of cells aggregated to form a nodule and capsule respectively, and finally undergoing melanization and removal from the body. The fine structure and the differential of hemocytes and fixed phagocytes are discussed in this paper.

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