The immune system in black tiger shrimp, Penaeus monodon Fabricius : I. Techniques on immunological assessment and blood component in black tiger shrimp, Penaeus monodon Fabricius
ระบบภูมิคุ้มกันโรคในกุ้งกุลาดำ : I. เทคนิคในการศึกษาระบบภูมิคุ้มกันโรค และองค์ประกอบ เลือดในกุ้งกุลาดำ

Kidchakan Supamattaya, Assoc. Prof., D. of Aquatic Sci., F. of Natural Resources, PSU.
Usanee Ekpanithanpong, Marine Shrimp Research Center, Muang, Songkhla
Toshiaki Itami, Assoc. Prof., D. of Applied Aquabiology, National Fisheries U., Shimonoseki, Japan
Jiraporn Kasornchandra, Marine Shrimp Research Center, Muang, Songkhla
Corresponding e-mail : skidchak@ratree.psu.ac.th

Grant : BIOTEC
Published : Songklanakarin J Sci Technol 2000, 22(Suppl.) : 567-580
Key words : immune system, blood parameters, black tiger shrimp, Penaeus monodon

The objectives of these studies were to develop appropriate techniques as standard method for studies of blood component and immune function in black tiger shrimp (Penaeus monodon

Fabricius). The studies comprise a study on techniques for collection and separation of hemocytes, application of continuous gradient centrifugation techniques for separation of hemocytes, immunological function of hemocytes and serum i.e. phagocytic activity, clearance ability of bacteria, bactericidal and virucidal activity in the serum, prophenoloxidase activating system, superoxide production of hemocytes and copper content in serum. The application of techniques in immunological and blood parameters stu-dies in black tiger shrimp are also discussed.

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