The specificity and sensitivity of serum immunoassay for anti-H-Pylori antibodies : Report of a preliminary result

Siriporn Ratanalert, Nurse., D. of Internal Medicine, F. of Medicine, PSU.
R. Wonsit, D. of Internal Medicine, F. of Medicine, PSU.
Teerha Piratvisuth, Assoc. Prof., D. of Internal Medicine, F. of Medicine, PSU.
Bancha Ovartlarnporn, Assoc. Prof., D. of Internal Medicine, F. of Medicine, PSU.
Corresponding e-mail : ptimmy@ratree.psu.ac.th

Published : J Gastroenterol Hepatol 1999, 14(Suppl.) : S71
Key words : anti-H-Pylori antibodies, CLO test

C-Urea breath test or serology are recommended in the screening of dyspepsia in many countries. Numerous types of Rapid office tests are currently available performance.
Obejective : To assess the sensitivity and specificity of One-step Hp test (OT) as compared wit rapid urease test (CLO test) (CLO).
Methods : All patients with age > 18 without previous treatment of PPI, antibiotic, Bismuth or NSAIDS within 30 days and required HP test by CLO were recruited from Feb. 1999 - Jul. 1999. Five ml. Of blood was sent to have OT done by one author (WR) without knowing the result of CLO. The CLO was read at 1-hr and 24-hr if the 1-hr reading was negative.
Results : 60 cases with the mean age of 51.88 and a range of 28-83 years were included. There were 41 males and l9 females. The diagnoses among the group were DU 19, GU 11, Duodenitis 9, gastritis 14, CA stomach 3, PU 1 and normal 3.33 were HP + ve by CLO. The result of OT compared with CLO was showed in the table below : 
						   		
                                                                     One step H.P test

								                
                                                                              Positive	              Negative            Total

  CLO test		Positive		19		17		36
		Negative		  5		19		24

		 Total		24		36		60
The OT has the sensitivity of 52.7%, the specificity of 79.6%, PPV of 79.6% and NPV of 52.7% compared with CLO.
Conclusions : The result in this study showed low sensitivity and specificity of the OT. The test is not suitable for screening dyspepsia patients.
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