Effect of disinfectants : chlorine, povidone iodine, on the viability of
Vibrio harveyi
ผลของสารเคมีฆ่าเชื้อ คลอรีน โพวิโดนไอโอดีน ต่อความอยู่รอดของ Vibrio harveyi
Somrak Panphon, D. of Sci., F. of Sci. and Tech., PSU.
Niyom Kamlangdee, School of Biology, Inst. of Sci., Walailuk U., Nakhon Si Thammarat
Presented : The 26th Congress on Science and Technology of Thailand, 18-20 October 2000,
Bangkok
Key words : Vibrio harveyi, disinfectant, chlorine, povidone iodine, viability
The marine pathogenic bacteria, identified as Vibrio harveyi, was isolated from infected
shrimps (Penaeus monodon Fabricius) and Luminous seawater collected from shrimp ponds. It was found that V. harveyi showed some significant characteristics including luminescent on Luminous media and a green and round colony on TCBS agar. The bacteria produced enzymes such as catalase, oxidase, amylase, gelatinase, and lipase and acid from fermentation of glucose. However, it did not produced gas from glucose fermentation. In addition, it could not grow in peptone water containing 0 and 10% of NaCl and at 5oC. The effects of disinfectants including chlorine and povidone iodine (PI) on V. harveyi growth were studied. It was found that V. harveyi (9.4x106 CFU/ml) were completely disinfected by 4 ppm of chlorine within 30 minutes. Unsteriled seawater (30 ppt) with V. harveyi
having total bacteia count of 1.3x107 CFU/ml were completely disinfected by 6 ppm of chlorine within 30 minutes. Povidone iodine at the concentration of 8 ppm (active ingredient) completely disinfected V. harveyi (9.2x106 CFU/ml) within 30 minutes. In addition, unsteriled seawater (30 ppt) with V. harveyi having total bacteria count of 1.9x107 CFU/ml were completely disinfected by 10 ppm (active ingredient) of PI within 30 minutes.
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