HLA phenotypes in donors and patients underwent kidney transplanta- tions and stem cell transplantations in Songklanagarind Hospital
HLA phenotypes ในผู้ป่วยและผู้บริจาคไตและไขกระดูกในโรงพยาบาลสงขลานครินทร์

Charuporn Promwong, D. of Pathology, F. of Medicine, PSU.
Wanwimon Yindee, Blood Bank, D. of Pathology, F. of Medicine, PSU.
Prapaporn Jitphakdee, Blood Bank, D. of Pathology, F. of Medicine, PSU.
Corresponding e-mail : pcharupo@ratree.psu.ac.th

Presented : Poster presentation in 16th Annually Academic Meeting of Faculty of Medicine, 16-18 August 2000, at Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla
Key words : HLA phenotype, HLA frequency, tissue transplantation

Introduction : HLA similarity between graft and host has very important role for engraftment in tissue transplantation. It has long been known that HLA genes are very polymorphism and the chance to find HLA identical donors is very few. The understanding of HLA phenotypes and the frequencies in certain population may be useful to find the potential donors for tissue transplantation.
Objective : To study HLA phenotypes and frequencies in donors and patients underwent kidney transplantation and stem cell transplantation in the Blood Bank of Songklanagarind Hospital.
Materials and methods : The HLA phenotypes of 112 donors and patients underwent either kidney transplantation or peripheral stem cell transplantation during November 1991 - June 1999 were reviewed. Fifty-seven subjects were tested HLA for kidney transplantation and 62 cases were tested for peripheral stem cell transplantation. The phenotyping of HLA-A, -B, -DR, -DQ were performed by standard microlymphocytotoxicity test.
Results : The first 3 highest phenotypes of each HLA antigens in total 112 subjects 
   
HLA phenotypes  A2 A24(9)  A11.1  B60(40)    B46  B13   DR12(5)   DR9   DR4  DQ6(1)    DQ7(3)
No. of subjects   48	45       38	       23	       22	19      26	       25	  24      39        35
      %	      40.3	37.8   31.9      19.3	    18.5	16.0  28.3	     27.2	26.1   44.3      39.8
Discussion and conclusion : From the study, there were slightly differences of HLA pheno-types in the subjects studied in the South compared with the study by the group in Siriraj Hospital (Chandanayingyong et al, 1995) which the highest antigens found were A11, A2/ B46, B40/ DR2, DR12/DQ1, DQ3. One of the factors that affect the engraftment was the mismatch HLA phenotype between donors and recipients, which resulted from the polymorphism characteristic of the genes. In contrast, being polymorphism is also very useful for identifying individuals. In conclusion the study of HLA phenotype frequency in certain population may be useful in both clinical and basic medical science.
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