Production and formulation of Trichoderma spp. biomass used to control leaf blight of Bambarra goundnut (Vigna subterranea) caused by Rhizoc-tonia solani in the South of Thailand
Ashara Pengnoo, D. of Earth Sci., F. of Natural Resources, PSU.
Mana Kanjanamaneesathian, Asst. Prof., D. of Pest Management, F. of Natural Resources, PSU.
Visanu Somsap, D. of Plant Sci., F. of Natural Resources, PSU.
Paranee Sawangsri, F. of Natural Resources, PSU.
Amornrat Chumthong, D. of Plant Sci., F. of Natural Resources, PSU.
Anong Nooduang, F. of Natural Resources, PSU.
Corresponding e-mail : pashara@ratree.psu.ac.th
Grant : National Biological Control Research Center. Southern Regional Center.
Presented : The 3rd International Symposium on Rhizoctonia (ISR 2000), 17-20 August 2000 National Chung Hsing University, Taichung, Taiwan (ROC)
Key words : Trichoderma spp. leaf blight, Vigna subterranea, Rhizoctonia solani, formulation, oil palm, biological control.
This project aims to formulate and produce an effective Trichoderma spp. biomass technology to control leaf blight disease in Bambarra groundnut caused by Rhizoctonia solani. This disease is a major disease in Southern Thailand because environmental conditions are suitable for rapid disease development. Production inputs such as fertilizer and fungicide applications for leaf blight control are minimal because this crop are cultivated as intercrop with other main crops poor soils by low income farmers. The Trichoderma spp. biomass has been produced with applicable technology which will be consequently transfer to farmers who can produce and formulate Trichoderma spp. biomass with basic requirement by their own. Trichoderma spp. biomass was initially produced by transferring the fungus to flask containing sterile mesocarp fiber of oil palm mixed with urea fertilizer (10:1, w/w). The fungus was incubated at room temperature at 26-30 oC for 7 days and then mixed with dolomite (pH 8.2 , 325 mesh) (1:1, v/v) in Waring blender. The formulation was stored in plastic bag and kept in an air tight plastic container and stored in the refrigerator at 6 oC. To assess the shelf life of the end product, colony forming unit (cfu) per gram of the formulation was carried out every 14 days. After 3 months of storage in the refrigerator, the formulation contained 1 x 10 10 5 cfu per gram. The further testing to determine longer shelf life has been studied. Research is in propers to test the developed Trichoderma spp in biomass and to determine its efficacy in controling leaf blight disease in pot and field experiments.
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