A survey on genetic variability of longkong (<I>Lansium domesticum</I> Corr.) seedlings by RAPD (Random Amplified Polymorphic DNA) technique

A survey on genetic variability of longkong (Lansium domesticum Corr.) seedlings by RAPD (Random Amplified Polymorphic DNA) technique
การศึกษาความแปรปรวนทางพันธุกรรมของต้นกล้าลองกอง (Lansium domesticum Corr.) ที่ได้จากการเพาะเมล็ดโดยใช้เทคนิค RAPD (Random Amplified Polymorphic DNA)

Charassri Nualsri, Asst. Prof., D. of Plant Sci., F. of Natural Resources, PSU.
Sompong Te-Chato, Assoc. Prof., D. of Plant Sci., F. of Natural Resources, PSU.
Mongkol Lim, Assoc. Prof., D. of Plant Sci., F. of Natural Resources, PSU.
Usa Choorak, D. of Plant Sci., F. of Natural Resources, PSU.

Grant : Prince of Songkla University
Published : Research Report
Key words : longkong, duku (Lansium domesticum Corr.), RAPD, genetic variability

Genetic variation of longkong and duku seedlings was determined by RAPD technique with 5 oligonucleotide primers; OPB-07 (GGTGACGCAG), OPC-05 (GATGACCGCC), OPC-08 (TGGACCGGTA), OPD-01 (ACCGCGAAGG) and OPD-03 (GTCGCCGTGA). In total 149 long-kong seedlings including 9 maternal plants, were analyzed. Identical DNA patterns were found, ex- cept 2 seedlings exhibited slightly different fragment pattern profiles and 49% of duku seedlings revealed genetic variation that differ from their maternal plants. Based on the results obtained from this study, it was concluded that longkong produces almost only nucellar seedlings while duku pro-duces 49% zygotic seedlings, indicating longkong can be propagated true-to-type by seed.

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