Screening and identification of antagonistic fungi Trichoderma spp.
for inhibition of important plant pathogens
การคัดเลือกสายพันธุ์และการศึกษาเพื่อจำแนกเชื้อราปฏิปักษ์ Trichoderma spp.
ที่มีศักยภาพในการควบคุมเชื้อราสาเหตุโรคพืชที่สำคัญ
Mana Kanjanamaneesathian, Asst. Prof., D. of Pest Management, F. of Natural Resources, PSU.
Anong Nooduang, National Biological Control Research Center, F. of Natural Resources, PSU.
Sakol Suwaluk, Electron Microscope Unit, D. of Pathology, F. of Medicine, PSU.
Corresponding e-mail : kmana@ratree.psu.ac.th
Grant : National Biological Control Research Center, Southern Regional Center, FNR,
PSU.
Published : Thai Agr Research J 2000, 18(1) : 4-16
Key words : biological control, Trichoderma spp
.
Antagonistic fungi Trichoderma spp. were isolated from forest and cultivated soils in the
southern region of Thailand. One hundred eighty-three isolates of Trichoderma spp. and Trichoderma-like fungi were obtained from the soil plate technique. All isolates were tested in vitro for inhibition
of mycelial growth of Phytophthora palmivora, Rhizoctonia solani and Sclerotium rolfsii. Cultivated soils were a better source of potential antagonistic fungi Trichoderma spp. than forest soils because they harbored isolates of Trichoderma spp. which were aggressive against three species of plant pathogens. Four isolates of Trichoderma spp. and two isolates of Gliocladium spp. had a potential as selected biological control agents. These potential fungal antagonists were identified using Scanning Electron Microscope (SEM) as Trichoderma harzianum (isolate no. 0123), Trichoderma viride (isolates no. 0140), and Gliocladium virens (isolates no. 0104 and 0138). Isolate no. 0015 and no. 0173 were an unidentified species.
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