Scanning electron microscopic examination of a pellet formulation of Bacillus megaterium and B. pumilus, antagonists of Rhizoctonia solani, and survival during storage

Mana Kanjanamaneesathian, Asst. Prof., D. of Pest Management, F. of Natural Resources, PSU.
Mana Kanjanamaneesathian, Asst. Prof., D. of Pest Management, F. of Natural Resources, PSU. Ashara Pengnoo, D. of Earth Sci., F. of Natural Resources, PSU.
Apinya Jantharangsri, Scientific Equipment Centre, PSU.
Ladda Nilratana, Technician, D. of Microbiology, F. of Sci., PSU.
Chirasak Kusongwiriyawong, D. of Pharmaceutical Tech., F. of Pharmaceutical Sci., PSU.
Corresponding e-mail : kmana@ratree.psu.ac.th

Grant : Government Budget and National Biological Control Research Center, Southern Regional Center
Published : World J of Microbiology & Biotechnology 2000, 16 : 523-527
Key words : Bacillus megaterium, B. pumilus, biological control, pellet formulation, Rhizoctonia solani, storage, survival

Bacterial formulations, produced using both Bacillus megaterium and B. pumilus individually with pharmaceutical technology, were formulated using a wet granular method. Viability testing in the laboratory revealed that bacterial populations rapidly declined during storage at room temperature (26-30^oC) for 6 months. The scanning electron microscope (SEM) was used to observe bacterial formulations. Both endospores and vegetative cells of B. megaterium and B. pumilus were detected on the formulation surfaces.

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