A protocol towards micropropagation of the piscicidal plant, Maesa
ramentacea A.DC.
Kamnoon Kanchanapoom, Assoc. Prof., D. of Biology, F. of Sci., PSU.
Kantamaht Boonvanno, The Natural History Museum, F. of Sci., PSU.
Corresponding e-mail : kkamnoon@ratree.psu.ac.th
Published : Science Asia 2000, 26 : 201-205
Key words : Maesa ramentacea A.DC., micropropagation, multiple shoots, piscicidal plant,
tissue culture
A tissue culture system was established from shoot tips of the piscicidal plant, Maesa ramentacea A.DC. Shoot proliferation was achieved with Murashige and Skoog (MS) medium supplemented with either BA, BA-Kinetin or BA-NAA combinations. Stocking of shoot cultures was obtained by repeating subculture in the same medium at 30-day intervals wherein the rate of multi-plication was maintained. For rooting, the individual shoot was implanted on root induction medium consisted of MS medium supplemented with 2 mg/l NAA. Within two weeks of incubation 100% rooting was evidenced. Complete plantlets were acclimatized and successfully transplanted to soil.
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