Tooth germ of rat in primary culture
การทดลองเลี้ยงเนื้อเยื่อตัวอ่อนของฟันหนู
Jaranpat Khaejornbut, D. of Oral Biology and Occlusion, F. of Dentistry, PSU.
E-mail : kjaranp@ratree.psu.ac.th
Grant : Government Budget
Published : Research Report
Key words : tooth germ of rat, primary culture, tissue cultured procedure
The main objective of this study is to test the "Tooth germ" tissue cultured procedure, to see
if those tooth germs can survive and continue their development for another 7 days. We took tooth germs of the mandibular first molar from the 10 days intra-uterus (i.u.) rats' embryo. These tooth
germs were then divided into 2 groups which the first group is a control while the second is an ex-perimental group. Then, specimens from the 1st group were put into the fixative immediately while specimens from the 2nd group were put into the cultured media tray and "incubated" for another 7
days inside a standard incubator. We prepared specimens from the control group using standard histology process and after 7 days incubation of the experimental group, we also have them arranged in the same method. Result from both groups were studied and compared under a light-microscope.
The result is a satisfaction one as tissue from the control group showed exactly the oral epithelium thickening period as the 10 day i.u. rat should. Among the cultured experimental tissues, they ad-
vance their development to the "BUD" stage where epithelium clearly proliferated down into the mesenchyme and, at the same time, mesenchymal cells did aggregate and surround the proliferating epithelium which the normally developed tooth germ of the 7 days old rat should. The successful
result will encourage the future research in this field of "epithelial-mesenchymal interaction during tooth development" which is still not clearly understood.
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