Effect of types and conditions of storage media on the vitality of human periodontal ligament cells
Wimonrat Jaturabundit, D. of Conservative Dentistry, F. of Dentistry, PSU.
Presented : The 4th Scientific Meeting of the Thai Dental Faculties Board, October 27-29,
1999, Rayong, Thailand
Key words : cell culture, human periodontal ligament, storage media
The purpose of this study is to determine the ability of various storage media to maintain viable human periodontal ligement (PDL) cells in vitro. Four different types of unsweetened milk available
in Thailand (pasteurized whole milk, pasteurized low fat milk, UHT whole milk and UHT low fat milk) Hank's Balanced Salt Solution and Dulbecco's Modification of Eagle's Medium were tested as storage media for periods of 2, 6 and 12 hours at 40oC (in refrigerator) and at room temperature (27oC - 32oC). PDL cells were cultured from healthy extracted human premolar teeth and plated into 96-well culture plates for cell viable evaluation. Two hundred ml of each tested storage media was replaced in each well for each different test condition. Cell viability was evaluated after treatment using neutral red vital staining assay and the absorbance values at 540 nanometre were determined. In addition, cells stored 6 and 12 hours in milk were also tested with respect to cell recovery. The results indicated that Dulbecco's medium was the most effective medium at all tested conditions and HBSS was the second. All types of milk products showed similar results in maintaining cell viability with
no statistically significant difference (p>0.05). Short storage time gave more viable cells than long time. With prolonged storage time, cells stored at 4oC showed higher viability than those stored at room temperature. The remaining cells after stored 12 hours in milk, could undergo mitosis. It is recommended that all types of milk products may be considered as suitable media for maintaining viable PDL cells of avulsed tooth before visiting the dentist as soon as possible.
BACK