Positive blood culture after 7 days of HIV positive patients
การตรวจพบเชื้อในเลือดของผู้ป่วย HIV หลังจากรายงานผลลบเมื่อเพาะเลี้ยงครบ 7 วัน
Sureerath Jealae, D. of Pathology, F. of Medicine, PSU.
Varaporn Laohaprertthisan, D. of Pathology, F. of Medicine, PSU.
Wanna Intasara, D. of Pathology, F. of Medicine, PSU.
Amporn Auanseng, D. of Pathology, F. of Medicine, PSU.
Urairat Kongmuang, D. of Pathology, F. of Medicine, PSU.
Corresponding e-mail : kurairat@ratree.psu.ac.th
Presented : The 16th Annual Academic Meeting, Faculty of Medicine, PSU., Hat Yai,
Songkhla, 10-18 August 2000
Key words : blood culture, HIV patients, fungus, Mycobacterium,
BacT/Alert automated system
Blood culture based on BacT/Alert automated blood culture system is incubated up to 7 days. No growth will be reported on day 7 of negative culture. Basically, most of the causative bacteria can be detected within 1-2 days.
Because of a high rate of disseminated infection with fungus and Mycobacterium frequently found in HIV infected patients, therefore the 7 day negative blood cultures were studied. A total of
172 samples of 7 day negative blood cultures were further investigated for fungal infection and mycobacterial infection by subculture on Sabouraud dextrose agar (SDA) and Lowenstein Jensen media (LJ), respectively.
Fifty of 172 (29%) was positive cultures. They were 34 Mycobacterium species (2 M. tuber-culosis, 3 M. avium intracellulare, 2 M. kansasii, 1 M. scorofulaceum, 11 Saprophytic mycobacterium and 15 were identifying), 16 Fungus (8 C. neoformans, 7 H. capsulatum and 1 P. marneffei).
This study has shown that 1) Fungus and Mycobacterium can be recovered in standard blood culture bottle. 2) Blood culture of HIV patients should be further investigated for fungus and Mycobacterium in routine culture.
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