Immunostimulant and vaccination in black tiger shrimp. Penaeus monodon Fabricius : IV The stimulatory effect of high and low molecule peptidoglycan (PG) on immune responses in black tiger shrimp, Penaeus monodon Fabricius

Immunostimulant and vaccination in black tiger shrimp. Penaeus monodon Fabricius : IV The stimulatory effect of high and low molecule peptidoglycan (PG) on immune responses in black tiger shrimp, Penaeus monodon Fabricius
สารกระตุ้นภูมิคุ้มกันและการใช้วัคซีนในกุ้งกุลาดำ : IV ผลของเปปติโดกลัยแคนโมเลกุลใหญ่ และเล็กต่อระบบภูมิคุ้มกันของกุ้งกุลาดำ

Mali Boonyaratpalin, Department of Fisheries, Kaset Klang, Bangkok
Kidchakan Supamattaya, Assoc. Prof., D. of Aquatic Sci., F. of Natural Resources, PSU.
Juadee Pongmaneerat, Department of Fisheries, Kaset Klang, Bangkok
Sitdhi Boonyaratpalin, Department of Fisheries, Kaset Klang, Bangkok
Yasuhiko Toride, Ajinomoto Co., Japan
Corresponding e-mail : skidchak@ratree.psu.ac.th

Grant : Ajinomoto Co. Inc. Thailand and BIOTEC
Published : Songklanakarin J Sci Technol 2000, 22(Suppl.) : 689-696
Key words : immunostimulant, peptidoglycan, superoxide anion, phenoloxidase, black tiger shrimp

High and low molecular peptidoglycan (hm-PG and lm-PG) extracted from Bifidobacterium thermophilum were mixed in shrimp feed at the levels of 0 and 0.1% for hm-PG and 0.0085% for lm-PG. Shrimps were fed with peptidoglycan (PG) supplemented diets for five weeks. Superoxide anion production from hemocyte of shrimp fed PG supplemented diet was higher than that of the control shrimp. Phenoloxidase activity in the cytoplasmic granules of hemocyte of shrimps fed test and con-trol was determined, but showed no significant differences.

The results on clearance ability of shrimp against bacteria in the hemolymph showed that the shrimps fed PG supplemented diets were able to eliminate 90% of bacteria whereas the control shrimps could eliminate only 50%. Moreover, shrimps fed with PG supplemented diets were more resistant to Vibrio harveyi infection than the control group with the survival rates of 61, 70 and 10% for shrimp that fed hm-PG, lm-PG supplemented diets and control diet, respectively. The results on superoxide anion production from the shrimp hemocyte and resistance to bacterial infection revealed that lm-PG was a better immunostimulant than hm-PG.

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