In vitro propagation of Gladiolus

Kantamaht Boonvanno, The Natural History Museum, F. of Sci., PSU.
Kamnoon Kanchanapoom, Assoc. Prof., D. of Biology, F. of Sci., PSU.
Corresponding e-mail : bkantama@ratree.psu.ac.th

Published : Suranaree J Sci Technol 2000, 7 : 25-29
Key words : axillary shoot formation, bud culture, Gladiolus, micropropagation, tissue culture

Multiple shoots of Gladiolus were initiated from axillary buds excised from corms. A single plant emerged on a medium consisting of the basal medium of Murashige and Skoog (1962, MS)+2.3 mM kinetin or MS+2.3mM NAA. In the presence of sole 2.3 mM NAA, the buds developed into callus. After prolonged culture in the same medium, rooting occurred without shoots. Multiple shoots were initiated from this callus when they were being transferred to MS medium containing 4.4 Benzyl-adenine (BA). Rooted shoots were obtained when multiple shoots were divided and subcultured to fresh medium. After transfer to pots in the greenhouse, the plantlet continued to grow with normal appearance.

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