Transferring of genuine characteristics from fragrance orchids to
commercial orchids
การถ่ายโอนลักษณะดีของกล้วยไม้หอมเข้าสู่กล้วยไม้เศรษฐกิจ
Punnee Asawatreratanakul, D. of Biochemistry, F. of Sci., PSU.
E-mail : apunnee@ratree.psu.ac.th
Grant : Government Budget
Published : Research Report
Key words : fragrance orchids, protoplast, Dendrobium Pompadour, hygromycin, kanamycin
Callus induction in Dendrobium Pompadour was conducted from culturing of seedling in vitro onto modified basal Murashige and Skoog (1962; MS) medium with various concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D) and kinetin under continuous light of about 2,000 lux for 16
hours under aseptic condition. The result showed that medium MS6 composed of the mixture of 2,4-D at concentration 2.5 mM and kinetin at concentration 2.5 mM was the best media. For Dendrobium scabrilingue callus formation was obtained when cultured on MS4 supplemented with 0.5 mM 2,4-D and 10.0 mM kinetin.
I have successfully tranferred plasmid which contained hygromycin and kanamycin resistance gene into Agrobacterium tumefaciens and then infected to plant tissue. The results showed that both infected Dendrobium Pompadour and Infected Dendrobium scabrilingue can grow well in the me-
dium which contain hygromycin and kanamycin.
The protoplasts were prepared from Dendrobium Pompadour and Dendrobium scabrilingue
in the buffer containing 1% macerozyme, 1% cellulase and 0.4 M sorbitol. From protoplast fusion experiment, the microscopic observation showed that the Dendrobium Pompadour can fuse with Dendrobium scabrilingue by using PEG 6000.
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