Pharmacokinetics of chlorhexidine in saliva
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Panomwat Amornphimoltham, D. of Oral Biology and Occlusion, F. of Dentistry, PSU.
Suwanna Jitpukdeebodintra, Asst. Prof., D. of Oral Biology and Occlusion, F. of Dentistry, PSU.
Sittichai Koontongkaew, Assoc. Prof., D. of Oral Biology and Occlusion, F. of Dentistry, PSU.
Corresponding e-mail : ksittich@ratree.psu.ac.th

Grant : Government Budget
Published : J Dent Assoc Thai 2000, 50 : 131-138
Key words : HPLC, chlorhexidine, saliva, pharmacokinetics, two-compartment model

A reversed-phase high-performance liquid chromatographic method was used for determina-tion of chlorhexidine in saliva. The mobile phase was acetonitrile-buffer (0.1 M disodium hydrogen phosphate, 0.005 M I-heptanesulfonic acid and 0.05 M triethyleneamine) (35 : 65, v/v) adjusted to pH 2.5 with phosphoric acid. The absorption peak of chlorhexidine was at 260 nm. The retention time for chlorhexidine was 5.92±0.14 min. The standard curve was linear from 0 to 40 mg/ml (r2 > 0.9). In a preliminary pharmaco kinetic study with 3 healthy volunteers, chlorhexidine remained in saliva after single use of 0.2% chlorhexidine mouthwashes for 2 hours. The salivary chlorhexidine clearance curves had the biphasic profile. It has been clearly demonstrated that clearance of chlorhexidine from the mouth is estimated by use the two-compartment open model.

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